Still tying to resolve this issue, can anyone point me in the right direction?

Added another email address for a different queue, pop account works great.  OTRS gets the email places in the right queue.  All out bound email, or when I generate “compose answer”  I get the following error?

ERROR: OTRS-CGI-77 Perl: 5.8.8 OS: linux Time: Wed Sep 26 14:03:06 2007

Message: Can't use from: user2@mydomain.com! Enable debug for more info!

Traceback (21487):
   Module: Kernel::System::Email::SMTP::Send (v1.13) Line: 86
   Module: Kernel::System::Email::Send (v1.29) Line: 494
   Module: Kernel::System::Ticket::Article::ArticleSend (v1.144) Line: 1610
   Module: Kernel::Modules::AgentTicketCompose::Run (v1.30) Line: 362
   Module: Kernel::System::Web::InterfaceAgent::Run (v1.22) Line: 670
   Module: /opt/otrs/bin/cgi-bin/index.pl (v1.81) Line: 47

Double checked the system address for the queues, and they are correct.  Anything else I can try?

Christian