I haven't tried it, but it seems reasonably possible to add a second authentication method. You'd just copy your current active directory setup and append 1 to the entries:
$Self->{'AuthSyncModule1'} = 'Kernel::System::Auth::Sync::LDAP';
$Self->{'AuthSyncModule::LDAP::Host1'} = 'ldap://ldap.example.com/';
$Self->{'AuthSyncModule::LDAP::BaseDN1'} = 'dc=otrs, dc=org';
$Self->{'AuthSyncModule::LDAP::UID1'} = 'uid';
$Self->{'AuthSyncModule::LDAP::SearchUserDN1'} = 'uid=sys, ou=user, dc=otrs, dc=org';
$Self->{'AuthSyncModule::LDAP::SearchUserPw1'} = 'some_pass';
$Self->{'AuthSyncModule::LDAP::UserSyncMap1'} = {
    # DB -> LDAP
    UserFirstname => 'givenName',
    UserLastname  => 'sn',
    UserEmail     => 'mail',
};
[...]

# AuthSyncModule::LDAP::UserSyncInitialGroups
# (sync following group with rw permission after initial create of first agent
# login)
$Self->{'AuthSyncModule::LDAP::UserSyncInitialGroups1'} = [
    'users',
];
http://forums.otrs.org/viewtopic.php?f=62&t=6619
http://forums.otrs.org/viewtopic.php?f=53&t=5567
http://forums.otrs.org/viewtopic.php?f=63&t=5177

On Mon, Feb 7, 2011 at 6:39 AM, Neil Simpson <nadsys@gmail.com> wrote:
Hi,

We have two active directory machines, primary and backup so to speak.

Currently OTRS is only configured to allow agents to login against the primary AD.  Is it possible to add a second authentication server to otrs in config.pm?

Has anyone tried this.  I simply can't just go and test it as it is a productive machine and needs downtime scheduled etc.

thank you

Neil

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